One Bucs coach tested positive and two other assistants were quarantined, sources said earlier this week.
The one unidentified Buccaneers assistant who tested positive is asymptomatic, sources said.
News of the Bucs players’ positive tests comes a day after Major League Baseball closed all spring training facilities in Florida and Arizona.
Florida recorded a record high of new COVID-19 cases Friday with 4,049. That same day, Tampa mayor Jane Castor implemented a mandatory ordinance for face masks to be worn in indoor public spaces.
Several Bucs players have been working out alongside new quarterback Tom Brady at Tampa’s Berkeley Prep.
ESPN’s Jenna Laine contributed to this report.